Praktikum 2 Penerapan Komputer

1Pengaturan untuk mengubah tombol kanan atau kiri : Jika di ceklis maka yang akan menjadi tombol ok adalah tombol kanan

Pengaturan kecepatan saat akan melakukan double klik : Jika digeser ke kiri maka double klik dapat dilakukan dengan kecepatan lemah

 

 

 

 

 

 

 

2

Pengaturan motion pada pointer : Jika digeser ke kanan maka ketika kursor digerakan akan mucul bayangan yang mengikutinya

 

 

 

 

 

 

 

 

 

3Pengaturan kecepatan kedipan kursor saat melakukan pengetikan misalnya pada Microsoft Word

Pengaturan kecepatan gerakan pointer : Jika digeser ke kiri maka gerakan yang dihasilkan gerakan yang sangat lambat

 

 

 

 

 

 

 

5Pengaturan besarnya volume speaker : Jika digeser ke kiri maka suara yang dihasilkan speaker akan sangat pelan

 

 

 

 

 

Pengembangan metodologi ekstraksi dan purifikasi DNA dari bulu burung Perkutut (Geopelia striata) untuk pemanfaatan studi sexing dan keragaman genetik

In the molecular studies of birds, the total DNA obtained from the extraction and purification of blood. Bird turtledove is very small and easy to stress so it is very risky if the blood is taken. Plumage may be other alternatives in obtaining DNA because it contains epithelial cells at their bases. The purpose of this research was to obtain a methodology of extraction and purification of DNA from the feathers of birds turtledove (Geopelia stiata) to study the utilization of sexing and genetic diversity. The method used consists of three kinds: 1) Digestion buffer-based method, 2) modification method using cetyl Trimethyl Ammonium bromide (CTAB buffer), and 3) the method of using tablets InhibitEX in CTAB buffer modification. The third method were divided into two treatments, the tablet was added before the addition of a solution of phenol or InhibitEX Before Phenol (IBP) and, the tablet was added after the addition of a solution of phenol or treatment InhibitEX After Phenol (IAP). The treatment period of soaking the samples in low-TE solution was during in 3 days and 14 days. The quality of DNA bands seen from the results of electrophoresis and spectrophotometer then compared with the control DNA from blood. Based on the purification result, the DNA was not obtained from Digestion buffer method. But, the DNA was obtained in CTAB buffer method, and the method using tablets InhibitEX been obtained DNA. Value averaging the results of the second method of DNA purity was lower (1.156) compared with the third method that is 3IBP (1.321) and 3IAP (1.551). PCR process was conducted as part to evaluate whether the DNA template (DNA template) from the extraction of fur can be amplified without a hitch or not. Based on the PCR test, 3IAP method has a high percentage (100%) compared with 3IBP method (50%) and method 2 (10%).

Sumber : Pradana, Zanuar Lingga

URL : http://repository.ipb.ac.id/handle/123456789/47576

Pengembangan metodologi ekstraksi dan purifikasi DNA dari bulu burung Perkutut (Geopelia striata) untuk pemanfaatan studi sexing dan keragaman genetik

In the molecular studies of birds, the total DNA obtained from the extraction and purification of blood. Bird turtledove is very small and easy to stress so it is very risky if the blood is taken. Plumage may be other alternatives in obtaining DNA because it contains epithelial cells at their bases. The purpose of this research was to obtain a methodology of extraction and purification of DNA from the feathers of birds turtledove (Geopelia stiata) to study the utilization of sexing and genetic diversity. The method used consists of three kinds: 1) Digestion buffer-based method, 2) modification method using cetyl Trimethyl Ammonium bromide (CTAB buffer), and 3) the method of using tablets InhibitEX in CTAB buffer modification. The third method were divided into two treatments, the tablet was added before the addition of a solution of phenol or InhibitEX Before Phenol (IBP) and, the tablet was added after the addition of a solution of phenol or treatment InhibitEX After Phenol (IAP). The treatment period of soaking the samples in low-TE solution was during in 3 days and 14 days. The quality of DNA bands seen from the results of electrophoresis and spectrophotometer then compared with the control DNA from blood. Based on the purification result, the DNA was not obtained from Digestion buffer method. But, the DNA was obtained in CTAB buffer method, and the method using tablets InhibitEX been obtained DNA. Value averaging the results of the second method of DNA purity was lower (1.156) compared with the third method that is 3IBP (1.321) and 3IAP (1.551). PCR process was conducted as part to evaluate whether the DNA template (DNA template) from the extraction of fur can be amplified without a hitch or not. Based on the PCR test, 3IAP method has a high percentage (100%) compared with 3IBP method (50%) and method 2 (10%).

Sumber : Pradana, Zanuar Lingga.2011. Institut Pertanian Bogor. Bogor

 

URL : http://repository.ipb.ac.id/handle/123456789/47576

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